polyclonal prm1 antibody  (Proteintech)

Journal: Cell Reports Medicine

Article Title: Decoding the pathogenesis of spermatogenic failure in cryptorchidism through single-cell transcriptomic profiling

doi: 10.1016/j.xcrm.2024.101709

Figure Lengend Snippet: Impaired spermatogenesis in CR (A) Left: UMAP plot of all germ cells ( n = 6,290 cells, 5 samples). The curve with the arrow represents the development trajectory of germ cells from SSCs to sperm. Right: separate UMAP plot of the CR group ( n = 3) and control ( n = 2). (B) Bar plot showing the cell ratio of each germ cell subset in (A). (C) IF staining of PRM1 in tissue sections of the CR group and control. Scale bars, 20 μm (PRM1) and 100 μm (merged). Control: testis samples from patients with obstructive azoospermia. (D) GO term enrichment of SSCs in downregulated genes. The size of the dot represents the gene count. The color of the dot represents the value of −log10 p value. (E) Left: IF staining of FGFR3 and ACTA2 in tissue sections of the CR group and control, showing the number of FGFR3+ cells significantly decreased in the CR group. Scale bar, 50 μm. Control: samples from adult males with obstructive azoospermia. Right: Quantification of the number of undifferentiated spermatogonia (FGFR3+) in a cross-section of each seminiferous tubule in different groups. Bars represent the mean with SD of 30 independent tubules in the CR group ( n = 3) and controls ( n = 3). The p value was calculated by Student’s t test. ∗∗∗∗ p < 0.0001, ∗∗ p < 0.01. (F) UMAP plot of 4 states for spermatogonia (merged SSCs and differentiating spermatogonia). Different colors represent different states. (G) Violin plots showing the expression of selected gene markers for different spermatogonium states. (H) Cell numbers of different spermatogonium states in the CR group and control. (I) The expression of classic cell-cycle-related genes in the CR group and control in different states. The color of the dot represents the average expression. (J) The expression of classic cell-cycle-related pathways in the CR group and control in different states.

Article Snippet: PRM1 Polyclonal antibody , Proteintech , Cat# 15697-1-AP; RRID: AB_2878168.

Techniques: Control, Staining, Expressing

Journal: Cell Reports Medicine

Article Title: Decoding the pathogenesis of spermatogenic failure in cryptorchidism through single-cell transcriptomic profiling

doi: 10.1016/j.xcrm.2024.101709

Figure Lengend Snippet:

Article Snippet: PRM1 Polyclonal antibody , Proteintech , Cat# 15697-1-AP; RRID: AB_2878168.

Techniques: Clinical Proteomics, Control, Software, Recombinant, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Role of Phosphorylated Gonadotropin-Regulated Testicular RNA Helicase (GRTH/DDX25) in the Regulation of Germ Cell Specific mRNAs in Chromatoid Bodies During Spermatogenesis

doi: 10.3389/fcell.2020.580019

Figure Lengend Snippet: Binding of GRTH to specific germ cell mRNAs in chromatoid bodies (CBs) isolated from wild-type (WT) and knock-in (KI) mice. Relative binding of Tnp1/2 , Prm1/2 , Grth , and Tssk6 mRNA to GRTH protein in CB of germ cells in the testes from WT and GRTH-KI mice. All statistical analyses were performed using Student’s t -test (* P < 0.05), and data represent mean ± SEM of three independent experiments in triplicates.

Article Snippet: The membrane was blocked with 5% skimmed milk powder in tris-buffered saline and then incubated with either of the following primary antibodies: affinity-purified anti-GRTH rabbit polyclonal antibody (1:500 dilution), anti-MVH/DDX4 polyclonal (1:500; cst#8761; Cell Signaling), mouse Argonaute/PIWI family RNA binding protein (MIWI) monoclonal antibody (CB control; 1:500; cst#6915; Cell Signaling), brain and muscle ARNT-like 1 (BMAL1) rabbit monoclonal antibody (1:500 dilution; cst#14020; Cell Signaling), circadian locomotor output cycles protein kaput (CLOCK) rabbit monoclonal antibody (1:500 dilution; cst#5157; Cell Signaling), Ybx3 mouse monoclonal antibody (1:500 dilution; LS-C105064; LS Bio), PRM1 rabbit polyclonal antibody (1:500 dilution; HPA055150; Millipore Sigma, St. Louis, MO, United States), and PRM2 rabbit monoclonal antibody (1:500 dilution; Sc-30172; Santa Cruz Biotechnology, Dallas, Texas).

Techniques: Binding Assay, Isolation, Knock-In